human alveolar type-ii pneumocyte cell line a549 Search Results


human alveolar type-ii pneumocyte cell line a549  (Bioarray Inc)
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Bioarray Inc human alveolar type-ii pneumocyte cell line a549
Levels of ACE2 protein ( A,D and E ) and enzymatic activity ( B and C ) in cells (A and D) and culture medium (E) in cultures of human alveolar type-II <t>A549</t> cells treated with spike protein alone or spike protein together with candesartan or captopril. Laser confocal microscopy showing colocalization ( H , yellow) of ACE2 (green, F ) and spike protein (red, G ) in cells after treatment of cultures with spike protein. * P <0.05 relative to untreated controls; # P <0.05 relative to cells treated with spike alone (one-way ANOVA with Student–Newman–Keuls Method post hoc test or Kruskal–Wallis one-way analysis of variance on ranks with Student–Newman–Keuls method post hoc test); scale bar (for F – H): 25 µm.
Human Alveolar Type Ii Pneumocyte Cell Line A549, supplied by Bioarray Inc, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Levels of ACE2 protein ( A,D and E ) and enzymatic activity ( B and C ) in cells (A and D) and culture medium (E) in cultures of human alveolar type-II A549 cells treated with spike protein alone or spike protein together with candesartan or captopril. Laser confocal microscopy showing colocalization ( H , yellow) of ACE2 (green, F ) and spike protein (red, G ) in cells after treatment of cultures with spike protein. * P <0.05 relative to untreated controls; # P <0.05 relative to cells treated with spike alone (one-way ANOVA with Student–Newman–Keuls Method post hoc test or Kruskal–Wallis one-way analysis of variance on ranks with Student–Newman–Keuls method post hoc test); scale bar (for F – H): 25 µm.

Journal: Clinical Science (London, England : 1979)

Article Title: Experimental data using candesartan and captopril indicate no double-edged sword effect in COVID-19

doi: 10.1042/CS20201511

Figure Lengend Snippet: Levels of ACE2 protein ( A,D and E ) and enzymatic activity ( B and C ) in cells (A and D) and culture medium (E) in cultures of human alveolar type-II A549 cells treated with spike protein alone or spike protein together with candesartan or captopril. Laser confocal microscopy showing colocalization ( H , yellow) of ACE2 (green, F ) and spike protein (red, G ) in cells after treatment of cultures with spike protein. * P <0.05 relative to untreated controls; # P <0.05 relative to cells treated with spike alone (one-way ANOVA with Student–Newman–Keuls Method post hoc test or Kruskal–Wallis one-way analysis of variance on ranks with Student–Newman–Keuls method post hoc test); scale bar (for F – H): 25 µm.

Article Snippet: Human alveolar type-II pneumocyte cell line A549 (CSC-C6236X, Creative Bioarray) was cultured in DMEM/F12 medium supplemented with 10% FBS, 2 mM L-glutamine (Sigma), 100 U/ml penicillin and 100 μg/ml Streptomycin.

Techniques: Activity Assay, Confocal Microscopy

Levels of proinflammatory cytokines (TNF-α, IL-6, CCL-2; A–C ) and ADAM17 enzymatic activity ( D and E ) in culture medium (A – C) and cells (D and E) in cultures of human alveolar type-II A549 cells treated with spike protein alone or spike protein together with candesartan or captopril. * P <0.05 relative to untreated controls; # P <0.05 relative to cells treated with spike alone (one-way ANOVA with Student–Newman–Keuls method post hoc test or Kruskal–Wallis one-way analysis of variance on ranks with Student–Newman–Keuls method post hoc test).

Journal: Clinical Science (London, England : 1979)

Article Title: Experimental data using candesartan and captopril indicate no double-edged sword effect in COVID-19

doi: 10.1042/CS20201511

Figure Lengend Snippet: Levels of proinflammatory cytokines (TNF-α, IL-6, CCL-2; A–C ) and ADAM17 enzymatic activity ( D and E ) in culture medium (A – C) and cells (D and E) in cultures of human alveolar type-II A549 cells treated with spike protein alone or spike protein together with candesartan or captopril. * P <0.05 relative to untreated controls; # P <0.05 relative to cells treated with spike alone (one-way ANOVA with Student–Newman–Keuls method post hoc test or Kruskal–Wallis one-way analysis of variance on ranks with Student–Newman–Keuls method post hoc test).

Article Snippet: Human alveolar type-II pneumocyte cell line A549 (CSC-C6236X, Creative Bioarray) was cultured in DMEM/F12 medium supplemented with 10% FBS, 2 mM L-glutamine (Sigma), 100 U/ml penicillin and 100 μg/ml Streptomycin.

Techniques: Activity Assay